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KMID : 0369820040340060465
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Jorunal of Korean Pharmaceutical Sciences
2004 Volume.34 No. 6 p.465 ~ p.469
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Protein-Coating Evaluation Method of Colloidal Gold Nanoparticles
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김미영/Kim MY
노상명/김정목/최한곤/김정애/오유경/Noh SM/Kim JM/Choi HG/Kim JA/Oh YK
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Abstract
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Colloidal gold nanoparticles might be of use as nano scale delivery systems of various therapeutic materials in the future. Recent studies have reported the feasibility of colloidal gold nanoparticles as gene delivery systems or protein delivery systems. In this study, we aimed to develop a short-step method useful for screening the optimal coating conditions of colloidal gold nanoparticles with proteins. We observed that colloidal gold nanoparticles have properties of changing its unique color when they were exposed to NaCl solution. Taking advantage of the color changing properties of colloidal gold nanoparticles, we applied the color testing method of colloidal gold nanoparticles solutions for evaluating the protein coating nature. Using bovine serum albumin as a model protein, we tested the protein coating of colloidal gold nanoparticles via the color change upon NaCl addition. The optimal coating concentration and coating conditions of colloidal gold nanoparticles with bovine serum albumin were fixed using the color testing methods. We suggest that the color testing method might be applied to optimize the coating condition of colloidal gold nanoparticles with other therapeutic proteins.
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KEYWORD
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Colloidal gold nanoparticles, Protein delivery systems, Protein coating, Color testing method
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